fig1

Figure 1. Schematic overview of the Caco-2/HepG2 co-culture system. Caco-2 cells were grown and differentiated on polycarbonate filter membranes (0.4 μm semipermeable pore diameter) for 21 days to recreate intestinal barrier. Then, Transwells were transferred on the top of confluent HepG2 cells seeded in multi-well plates and treated with endotoxin (LPS) and/or palmitic and oleic acids (FFAs) to mimic gut-liver interplay during NAFLD onset. NAFLD: Nonalcoholic fatty liver disease.