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Figure 1. scRNA-seq reveals genes differentially expressed in cytotoxic cells between sAH and HC. (A) Schematic showing how NK cells recognize activated target cells and how NK cells respond. (B) Venn Diagram showing genes commonly upregulated in sAH between NK cells and four subtypes of CD8 T cells: Naïve, Memory, and two distinct clusters of Effector cells. (C) Venn Diagram showing genes commonly downregulated in sAH between NK cells and effector CD8 T cells. (D) Violin plots showing expression of major C-type lectin receptors (Left) and components of cytolytic granules (Right). * = P < 0.05. (E and F) Correlation analysis to see how genes in NK cells are coordinately expressed at baseline for healthy control (E) and sAH (F). Blue squares indicate genes with highly correlated expression, while red squares indicate anti-correlated expression (see “METHODS”). sAH: Severe alcohol-associated hepatitis; HC: healthy controls.