fig3

Figure 3. NK-cell have reduced cytolytic granules in sAH. Flow cytometry of PBMCs from different metabolic liver disease patients to assess protein expression of granzyme B and perforin in NK cells and CD8 T cells. (A) NK cells, NKT-cells, and T-cells were defined by the expression of CD56 (NK cells), CD3 (T-cells), or both (NKT-cells). CD8 T cells were identified by the expression of CD8. Then perforin (PRF1), granzyme A (GZMA), and granzyme B (GZMB) were measured by median fluorescence intensity (MFI) and % positive cells. (B) MFI and % positive cells were measured for PRF1, GZMA, and GZMB within each disease. Error bars indicate standard deviation, * = P < 0.05, ^# = P < 0.05 compared to all groups. sAH: Severe alcohol-associated hepatitis.